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Target Binding Dissociation Constants (K D ) of Antibodies Upon Stress in PBS, pH 7.4, at 40°C. #

Journal: Pharmaceutical Research

Article Title: In vitro Stability Study of a Panel of Commercial Antibodies at Physiological pH and Temperature as a Guide to Screen Biologic Candidate Molecules for the Potential Risk of In vivo Asparagine Deamidation and Activity Loss

doi: 10.1007/s11095-025-03825-3

Figure Lengend Snippet: Target Binding Dissociation Constants (K D ) of Antibodies Upon Stress in PBS, pH 7.4, at 40°C. #

Article Snippet: The binding affinity of golimumab upon PBS stress for 28 d was almost unchanged by MSD and about threefold weaker by Biacore compared to unstressed golimumab ( , Supplementary Table , Supplementary Fig. ).

Techniques: Binding Assay

Inhibitory Effects of OMD and OMDI on α 2A -Adrenergic Receptor, β-Adrenergic Receptors, EP2 Receptor, and the Enzymatic Activities of CA1 and CA2

Journal: Translational Vision Science & Technology

Article Title: Ocular Tissue Distribution of Omidenepag Isopropyl in Rabbits and Cynomolgus Monkeys

doi: 10.1167/tvst.13.11.6

Figure Lengend Snippet: Inhibitory Effects of OMD and OMDI on α 2A -Adrenergic Receptor, β-Adrenergic Receptors, EP2 Receptor, and the Enzymatic Activities of CA1 and CA2

Article Snippet: Assays measuring the binding of OMD to EP2 receptor were performed by Sekisui Medical Co., Ltd. (Tokyo, Japan), and OMD and OMDI binding assays were performed by Eurofins Panlabs Discovery Services Taiwan, Ltd. (New Taipei City, Taiwan), as previously described.

Techniques: Inhibition